ISSN: 2459 - 1777 | E-ISSN 2587-0394
Carvacrol protects against paclitaxel-induced retinal and optic nerve cytotoxicity: a histopathological study [Beyoglu Eye J]
Beyoglu Eye J. Ahead of Print: BEJ-63825 | DOI: 10.14744/bej.2020.63825

Carvacrol protects against paclitaxel-induced retinal and optic nerve cytotoxicity: a histopathological study

Emine Çinici1, Nilay Dilekmen2, Zerrin Kutlu3, Büşra Dinçer4, Özkan Çinici5, Hilal Balta6, İlknur Çalık6
1Department of Eye Disease, Ataturk University, Erzurum, Turkey
2Palandoken State Hospital, Department Of Ophtalmology, Erzurum, Turkey
3Department Of Biochemistry, Ataturk University Faculty Of Pharmacy, Erzurum, Turkey
4Department Of Pharmacology, Erzincan Binali Yildirim University Faculty Of Pharmacy, Erzincan, Turkey
5Department Of Internal Medicine, Regional Training And Research Hospital, Erzurum, Turkey
6Department Of Pathology, Regional Training And Research Hospital, Erzurum, Turkey

INTRODUCTION: Carvacrol (CV) is a phenolic monoterpenoid found in essential oils of aromatic plants. In the present study, we investigated the potential protective effect of CV against paclitaxel (PTX)-induced retinal and optic nerve cytotoxicity in rats.
METHODS: 18 male adult Wistar albino rats (250-400g) were randomized into 3 equal groups comprising 6 animals in each. Group-1 (control group) received intraperitoneal (i.p.) saline solution (0.5 ml/200 g) weekly for 4 weeks. Group-2 received i.p. PTX (5mg/kg) while Group-3 received i.p. CV (25mg/kg) 30 minutes after i.p. PTX (5mg/kg) weekly for 4 weeks. At the end of the experimental period retinal and optic nerve tissues were evaluated histopathologically.
RESULTS: All retinal specimens in Group-1 (control) were histopathologically normal. In Group-2 (PTX) all eyes (6/6) demonstrated increased retinal vascularity and rosette-like structures in the outer nuclear layer while in Group-3 (PTX-CV), all eyes (6/6) demonstrated normal retinal vascularity and absence of rosette-like structures. All optic nerve specimens in Group-1 (control) were histopathologically normal. In Group-2 (PTX), all eyes (6/6) demonstrated severe vacuolization and reduction in the number of astrocytes and oligodendrocytes in optic nerve specimens while 3 eyes (3/6) demonstrated marked single cell necrosis. None of the eyes in Group-3 (PTX-CV) demonstrated either vacuolization or reduction in the numbers of astrocytes and oligodendrocytes. No remarkable single cell necrosis was observed in optic nerve specimens of Group-3 (PTX-CV).
DISCUSSION AND CONCLUSION: Our histopathological findings demonstrated the protective role of CV against PTX-induced cytotoxicity. CV might be a promosing molecule in counteracting oxidative stress-based cytotoxicity in the field of retinal and optic nerve disorders.

Keywords: paclitaxel, carvacrol, paclitaxel-induced cytotoxicity, oxidative stress, antioxidant



Corresponding Author: Emine Çinici, Türkiye
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