ISSN: 2459 - 1777 | E-ISSN 2587-0394
Volume : 6 Issue : 1 Year : 2021

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The Inhibitory Effect of Capparis Ovata Polysaccharides on Cultured Pterygium Fibroblasts [Beyoglu Eye J]
Beyoglu Eye J. 2021; 6(1): 1-6 | DOI: 10.14744/bej.2021.60352

The Inhibitory Effect of Capparis Ovata Polysaccharides on Cultured Pterygium Fibroblasts

Erkut Küçük1, Işıl Aydemir2, Kurşad Zor1, Oktay Özkan3
1Department of Ophthalmology, Nigde Omer Halisdemir University Faculty of Medicine, Nigde, Turkey
2Department of Histology and Embryology, Nigde Omer Halisdemir University, Faculty of Medicine, Nigde, Turkey
3Department of Pharmacology, Nigde Omer Halisdemir University Faculty of Medicine, Nigde, Turkey

INTRODUCTION: Pterygium recurrence after removal surgery is an important problem. Polysaccharides obtained from Capparis species have been shown to possess various biological properties, including anti-tumor activity. This study was an investigation of the effect of Capparis ovata polysaccharides on cultured pterygium fibroblasts and a comparison with the effects of mitomycin C (MMC).
METHODS: Pterygium tissue samples were obtained during excision surgery from 3 patients with primary pterygium, and fibroblasts were isolated. Pterygium fibroblast cultures and L929 cell cultures were treated with different concentrations of MMC and Capparis ovata polysaccharides. Cell proliferation and migration were evaluated.
RESULTS: An MTT assay revealed that cell viability decreased with increasing concentrations of Capparis ovata polysaccharides in both cell types. MMC also inhibited the proliferation of both cell types. A scratch-wound assay indicated that both Capparis ovata polysaccharides and MMC molecules reduced proliferation and migration in the pterygium fibroblasts and L929 cells.
DISCUSSION AND CONCLUSION: The in vitro Capparis ovata polysaccharide inhibition of proliferation and migration of pterygium fibroblasts was similar to that of MMC. The results of this study suggested that Capparis ovata polysaccharides may be a valuable candidate drug to treat pterygium.

Keywords: Capparis ovata, fibroblast, mitomycin C, polysaccharide, pterygium

Erkut Küçük, Işıl Aydemir, Kurşad Zor, Oktay Özkan. The Inhibitory Effect of Capparis Ovata Polysaccharides on Cultured Pterygium Fibroblasts. Beyoglu Eye J. 2021; 6(1): 1-6

Corresponding Author: Erkut Küçük, Türkiye
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