Beyoglu Eye J. 2023; 8(2): 110-114 | DOI: 10.14744/bej.2023.16779

Histopathological View of Benign Essential Blepharospasm: Orbicularis Oculi Hormone Receptor Levels

Kübra Þerefoðlu Çabuk¹, Ganime Çoban², Gülay Yalçýnkaya Çakýr³, Zeynep Sezal Þerefoðlu², Þenay Aþýk Nacaroðlu⁴, Gamze Öztürk Karabulut⁵, Korhan Fazýl^6
1University of Health Sciences Beyoglu Eye Training and Research Hospital, Istanbul, Türkiye
²Department of Pathology, Bezmialem Vakif University, Faculty of Medicine, Istanbul, Türkiye
³Department of Ophthalmology, Patnos State Hospital, Agri, Türkiye
⁴Department of Ophthalmology, Istanbul Medipol University, Istanbul, Türkiye
⁵Department of Ophthalmology, Arel University, Memorial Hospital, Istanbul, Türkiye
⁶Oculoplastic and Orbital Surgery and Ocular Oncology Center, Istanbul, Türkiye

OBJECTIVES: Benign essential blepharospasm (BEB) is a focal dystonia characterized by involuntary contractions of the orbicularis oculi and periocular muscles. We aimed to investigate the effects of muscle receptor levels on the etiopatho-genesis of blepharospasm by evaluating the orbicularis oculi estrogen receptor (ER) and androgen receptor (AR) levels.
METHODS: Four blepharospasm patients (2 females and 2 males) who underwent upper lid blepharoplasty and/or orbicu-laris myomectomy and 4 healthy cases (2 females, 2 males) that had upper lid blepharoplasty were included. The pretarsal, preseptal, and orbital parts of the orbicularis muscles of the patients who underwent orbicularis myomectomy and the waste muscle tissue materials taken from the preseptal orbicularis muscles of the patients who had only upper blepharo-plasty were analyzed. Immunohistochemical staining was performed with estrogen alpha and androgen.
RESULTS: In healthy men, the orbicularis oculi muscle was stained with ER at a moderate intensity and with AR at a high intensity. In men with blepharospasm, the orbicularis oculi were not stained with ER at all, but at a high intensity with AR. In healthy women, the orbicularis oculi were stained with ER and AR at a high intensity (>50%). In women with ble-pharospasm, the staining intensities of both receptors were moderate.
DISCUSSION AND CONCLUSION: We determined a decrease in ER and AR in females and almost the absence of ER in males with BEB. This decrease in ER may be associated with a functional abnormality in mitochondria and the decrease in hormonal receptors may be associated with sarcopenia in orbicularis oculi muscle fibers.

Keywords: Androgen receptor, benign essential blepharospasm, estrogen receptor, hormone receptor, orbicularis oculi

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