Beyoglu Eye J. 2023; 8(1): 50-54 | DOI: 10.14744/bej.2023.08931

The Prevalence of Positive Donor Corneoscleral Rim Culture and its Association with Ocular Infection After Transplantation

Ruveyde Bolac¹, Ece Turan Vural¹, Elvin Yildiz², Gizem Kaya³, Sebahat Aksaray^4
1Department of Ophthalmology, University of Health Sciences, Haydarpasa Numune Training and Research Hospital, Istanbul, Türkiye
²North American Lasik and Eye Surgery Center, Dubai, United Arab Emirates
³Department of Ophthalmology, Gazipasa State Hospital, Antalya, Türkiye
⁴Department of Microbiology, University of Health Sciences, Haydarpasa Numune Training and Research Hospital, Istanbul, Türkiye

OBJECTIVES: The aim of the study was to determine the prevalence of positive corneoscleral donor rim cultures and to report keratitis and endophthalmitis after keratoplasty.
METHODS: Eye bank records and medical records of patients who underwent keratoplasty between September 1, 2015, and December 31, 2019, were retrospectively reviewed. Patients who had routine donor-rim culture taken during surgery and followed up for at least 1 year in the post-operative period were included in the study.
RESULTS: A total of 826 keratoplasty procedures were performed. A total of 120 (14.5%) cases had a positive donor cor-neoscleral rim culture. Positive bacterial cultures were obtained from 108 (13.7%) of the donors. Bacterial keratitis was observed in one patient (0.83% of recipients) who had a positive bacterial culture. Positive fungal cultures were obtained from 12 (1.45%) donors, of whom one (8.33% of recipients) developed fungal keratitis. Endophthalmitis was observed in one patient whose culture result was negative. Both bacterial and fungal culture results were similar in penetrating and lamellar surgical procedures.
DISCUSSION AND CONCLUSION: Although the donor corneoscleral rims have a high positive culture result, the rate of bacterial keratitis and endophthalmitis is low, the risk of infection is high in patients with a fungal positive donor rim. Closer follow-up of patients with fungal positive donor corneo-scleral rim result and initiation of aggressive antifungal treatment when infection occurs will be beneficial.

Keywords: acterial keratitis, Endophthalmitis, fungal keratitis, keratoplasty, positive donor rim culture

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