ISSN: 2459 - 1777 | E-ISSN 2587-0394
Volume: 4
Issue: 1
Year: 2019
The evaluation of retinol palmitate against photokeratitis in rat eyes exposed to ultraviolet B radiation [Beyoglu Eye J]
Beyoglu Eye J. Ahead of Print: BEJ-51523 | DOI: 10.14744/bej.2019.51523  

The evaluation of retinol palmitate against photokeratitis in rat eyes exposed to ultraviolet B radiation

Mehmet Caner Filizay1, Nesrin Buyuktortop Gokcinar2, Varol Sahinturk3, Setenay Oner4, Sedat Kaçar3, Zafer Onaran2, erhan yumusak2
1Eskisehir State Hospital, Eskişehir, Turkey
2Kırıkkale University, Faculty of Medicine, Department of Ophthalmology, Kırıkkale, Turkey
3Eskişehir Osmangazi University, Faculty of Medicine, Department of Histology and Embryology, Eskisehir, Turkey
4Eskişehir Osmangazi University, Faculty of Medicine, Department of Biostatistics, Eskisehir, Turkey

INTRODUCTION: Acute exposure to ultraviolet B radiation causes photokeratitis. Retinol palmitate is known to have antioxidant properties and improve corneal healing. The aim of our study was to evaluate the efficacy of topical retinol palmitate against phototoxic keratitis in rats.
METHODS: Rat eyes were exposed to 1 J/cm2 dose of 311 nm ultraviolet B radiation, and recruited into 4 study groups. RP-5 group (n=7) received topical 250 IU/g ointment containing retinol palmitate, and Sham-5 group (n=7) received only the vehicle base component of the ointment, 5 minutes after the exposure. RP-120 group (n=7), received topical retinol palmitate and Sham-120 group (n=7) received vehicle alone, 120 minutes after the exposure. Eyes were enucleated 24 hours after the exposure and stained with hematoxylin and eosin for histopathological examination and terminal deoxynucleotidyl transferase dUTP nick end labeling assay for detection of apoptosis.
RESULTS: There was no statistically significant difference in the mean corneal epithelial thickness between the RP-5 group and Sham-5 group (p=0.369). There was no significant difference between the RP-120 and the Sham-120 groups, either (p=0.765). There was no significant difference between the timing of administration of retinol palmitate in terms of the mean corneal epithelial thickness (p=0.608). Apoptotic cell scores were not significantly different between corneas that received retinol palmitate versus vehicle alone ( p=0.530, p=0.107).
DISCUSSION AND CONCLUSION: Topical administration of a single dose of retinol palmitate was not superior to vehicle alone against the photokeratitis model produced using 1 J/cm2 of narrowband ultraviolet radiation on rats.

Keywords: Apoptosis, cornea, photokeratitis, retinol palmitate, ultraviolet, vitamin A




Corresponding Author: Nesrin Buyuktortop Gokcinar, Türkiye


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