ISSN: 2459 - 1777 | E-ISSN 2587-0394

Quick Search




Treatment of corneal alkali burn with chestnut honey, royal jelly, and chestnut honey-royal jelly combination [Beyoglu Eye J]
Beyoglu Eye J. Ahead of Print: BEJ-29290 | DOI: 10.14744/bej.2019.29290

Treatment of corneal alkali burn with chestnut honey, royal jelly, and chestnut honey-royal jelly combination

Kursat Atalay1, Kubra Serefoglu Cabuk2, Ahmet Kırgız2, Aysel Kara Caglar3
1Istanbul Saglik Bilimleri University, Bagcilar Training And Research Hospital, Ophthalmology Department
2Istanbul Saglik Bilimleri University, Beyoglu Resat Belger Eye Training And Research Hospital, Ophthalmology Department
3Istanbul Saglik Bilimleri University, Bagcilar Training And Research Hospital, Pathology Department

INTRODUCTION: The alkaline burn of the cornea usually results in a decreased vision with opacification. Here, we investigated the potential role of endemic chestnut honey (CH) and royal jelly on corneal healing after an alkaline burn.
METHODS: We created an alkaline burn on the center of the corneas of 4 groups of Wistar rats by applying a round filter paper soaked with 1 N NaOH for 30 seconds. The animals were treated with RJ, CH, RJ-CH combination, and Na-Hyaluronate (Na-HA) eye drops. We performed a serial evaluation with anterior segment photography on the first, 7th, and 14th days of the experiment. Pathologic examination conducted with hematoxylin and eosin stains and immunostaining for SMA and α4β1 integrin. We evaluated the corneal healing process with a scoring system which was estimating the degree of corneal edema, the size of the corneal ulcer, and limbal hyperemia.
RESULTS: There was no statistically significant difference between groups on the first, 7th, and 14th days concerning the healing scores (P=0.88, P=0.06, P=0.80; respectively). However, there were significantly better scores in repeated measures of CH (P=0.012) and RJ-CH (P=0.00) groups. The RJ group and Na-HA group did not show a significant difference at repeated measures (p=0.19 and P=0.10; respectively). The α4β1 integrin levels on immunostaining show a significant difference among groups on the 14th day (P=0.002).
DISCUSSION AND CONCLUSION: We found better corneal healing after treatment with the RJ-CH containing eye drops in terms of corneal healing sore and α4β1 integrin staining.

Keywords: Cornea, corneal alkaline burn, chestnut honey, royal jelly, smooth muscle antigen, α,, 4β1 integrin



Corresponding Author: Kursat Atalay, Türkiye
LookUs & Online Makale